Louise Juliana of Orange-Nassau (31 March 1576 in Delft – 15 March 1644 in Königsberg) was a countess of the Palatinate by marriage to Frederick IV, Elector Palatine, and regent during the minority of her son from 1610 until 1611. She was the eldest daughter of William of Nassau, Prince of Orange and his third spouse Charlotte de Bourbon-Montpensier.
Louise Juliana was the first Dutch born member of the House of Orange-Nassau. After her father was murdered in 1584, she and some of her five sisters were raised by their stepmother Louise de Coligny.
On 23 June 1593, Louise Juliana married Frederick IV, Elector Palatine of the Rhine.
After the death of her husband in 1610, she ruled in the name of her son Frederick V, known as "the Winter King."
Her regency was discontinued in 1611.
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